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ScienceDaily (Nov. 29, 2012) Chromatin remodeling -- the packaging and unpackaging of genomic DNA and its associated proteins -- regulates a host of fundamental cellular processes including gene transcription, DNA repair, programmed cell death as well as cell fate. In their latest study, scientists at the Stowers Institute for Medical Research are continuing to unravel the finicky details of how these architectural alterations are controlled.

Through a series of biochemical experiments, Stowers Investigators Ron Conaway, Ph.D., and Joan Conaway, Ph.D., and their team discovered that chromatin remodeling enzyme and suspected oncogene ALC1 (short for Amplified in Liver Cancer 1) is activated through an unusual mechanism: Its shape shifts in the presence of its activators. Their finding identifies a new instrument in cells' molecular repertoire of chromatin-remodeling tools and a potential cancer therapeutic target.

One of the main tasks of chromatin remodeling enzymes, Ron Conaway explains, is "to make DNA accessible so events like repair and gene transcription can occur." Postdoctoral research associate and first author Aaron Gottschalk, Ph.D., previously figured out that ALC1 required protein partners to activate its remodeling function. Publishing in an upcoming issue of the Journal of Biological Chemistry, he dissects the mechanism by which this occurs.

ALC1 and its ilk have a common protein domain, SNF2, that uses the energy of ATP hydrolysis to move nucleosomes -- the basic repeating units of chromatin -- around, in a process called nucleosome sliding.

"I was intrigued because ALC1 has a unique macrodomain not found on any other SNF2 family member," Gottschalk says. His interest was further piqued when he found that while most ATP-dependent chromatin remodelers function as large multi-protein complexes, ALC1 appeared to work by itself. At the same time, where most of its group-happy family members readily demonstrated nucleosome sliding activity in vitro, ALC1 was not only a lone ranger but also "completely dead on its own."

Gottschalk deduced that ALC1 may function independently, but it needs a boost from a couple of sidekicks: PARP1, an enzyme that responds to several kinds of DNA damage; and NAD+, the substrate by which PARP1 transfers chains of poly (ADP-ribose) onto itself and other target proteins, in a process called PARylation.

Only when PARP1 and NAD+ are on the scene does ALC1 spring into action, altering the accessibility of DNA by shifting nucleosomes around. Gottschalk's earlier findings were published in the Proceedings of the National Academy of Sciences in June 2009.

"We then extended this research," Conaway says, "and the upshot of our recent JBC paper is that ALC1 is likely activated through a series of physical interactions. ALC1's unique macrodomain can bind PAR, and protein-protein interactions also occur between ALC1 and PARP1." Gottschalk and coauthor Rushi Trivedi, a graduate student in the Biochemistry & Molecular Biology department at KU Medical Center, developed a novel footprinting assay that enabled this observation. Rather than merely activating ALC1 and moving on, the researchers found that the trio of PARP1, NAD+ and ALC1 hangs out in a stable complex.

"So PARylated PARP1 and NAD+ are allosteric effectors -- by binding to ALC1, they alter its state from dormant to active," Conaway says. "It's an interesting mechanism that's different from how most other chromatin remodelers work. It may also help explain other evidence that PARP1 has the ability to rearrange nucleosomes and reorganize chromatin; this could be one way by which PARP1 exerts its influence."

Apart from its role in modifying chromatin structure, not much is currently known about ALC1. It's regarded as a possible oncogene, being found in excess in hepatocellular carcinoma cells and because overexpression of ALC1 in mice induces spontaneous tumors.

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Chromatin remodeling: Activating ACL1 with a little help from 'friends'

HDR Remodeling, a remodeling contractor in the East Bay, recently received two awards during the annual "Remmie" Awards Dinner on Nov. 14.

Berkeley, CA (PRWEB) November 28, 2012

"The Bay Area is home to some of the most talented design and construction professionals in the country. We are proud that our company has been recognized for excellence from a field of such talented individuals and highly professional companies," Jim Tibbs, creative director of HDR Remodeling, said.

The Remmie Awards are judged by a team of four industry-leading professionals who select the winning projects based on creative problem solving, functional and aesthetic enhancements, craftsmanship, and innovative use of materials. The two winning kitchen designs from HDR Remodeling were completed in-house by Jim Tibbs and Lucy Chia Lu Kuo, designer, and executed under the management of Philip and Anderson and Brian MacRae, president and production manager of HDR Remodeling. HDR Remodelings two projects will be entered into the national competition, which is held in early 2013.

The San Francisco Bay Area chapter of NARI includes the cities of San Francisco, Berkeley, Oakland, Piedmont and the surrounding East Bay cities, as well as the North Bay up to and including Santa Rosa.

The competition within the Bay Area remodeling industry is fierce, based on the large number of very talented professionals and outstanding companies that operate in this area. For this reason, it is a major honor for a company to be singled out for an award. It is also a great way for the general public to be introduced to the work of the local leaders in the industry.

The mission of the San Francisco Bay Area chapter of NARI is to build an interactive community of general contractors, trade contractors, specialty contractors, designers, suppliers and affiliated professionals within the remodeling industry.

For more information about any of HDR Remodelings services, call 510-984-3755 or visit the home remodeling companys website at http://www.hdrremodeling-eastbay.com. HDR Remodeling's office is located at 2952 Sacramento St. in Berkeley.

About HDR Remodeling

HDR Remodeling is dedicated to providing clients with personalized remodeling services to give them healthier and more beautiful living spaces at reasonable budgets. The company is certified with Build It Green as a remodeling contractor and has also been awarded "Green Check" by the Sierra Club Green Home Initiative. HDR Remodeling designs and transforms homes to improve the comfort of clients while integrating green building practices.

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East Bay Home Remodeling Company HDR Remodeling Received Awards at "Remmie" Awards Dinner

Originally published December 1, 2012 at 8:46 AM | Page modified December 1, 2012 at 8:48 AM

If youre considering remodeling but cant decide if its worth going ahead, here are some things to consider.

1. Is it truly necessary or are you just trying to keep up with the neighbors? Sometimes a big remodeling job is so expensive you might be better off moving to a newer home.

2. Consider all the possibilities. Think about the cost. Can you afford it? Is your income secure? Remember that once you start, you cant turn back. A good architect and a good building contractor are worth the money, so dont skimp when it comes to choosing help.

3. Consider how long you intend to stay in this house. If you plan to stay for the rest of your life, then remodeling is a good idea. If you are planing to stay less than five years, you might consider simple improvements.

4. Remodeling is a messy, inconvenient and frustrating process filled with large and small disappointments. It will take longer than estimated. It will cost more as unforeseen costs appear. Prepare yourself.

By Rosemary Sandez Friedmann

Scripps Howard News Service

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Pros, cons of remodeling

CHESTER, Pa., Nov. 30, 2012 /PRNewswire/ --Power Home Remodeling Group, one of the nation's largest home remodeling companies, was included on the Boston Globe's 2012 Top Places to Work list. The Top Places to Work program identifies leading businesses in the state of Massachusetts based upon results from employee surveys.

Employees based at Power's Marlborough, MA location completed a detailed survey about their jobs including questions related to team effectiveness, retention, employee alignment with company goals and manager effectiveness citing the company's mission, ethics and positive culture as reasons why Power should be named one of Massachusetts' top employers.

"We are thrilled that our Marlborough employees have helped us achieve this honor," said Asher Raphael, Power's Chief Strategy Officer. "We strive to create an environment that encourages employees to feel connected and invested in the company while delivering an exceptional home improvement experience to customers, and it's reassuring to know that they appreciate and embrace our culture."

Power, which was recently named to Glassdoor's "Top 25 Companies for Career Opportunities" list, provides all employees with a variety of comprehensive customer service training programs that include site visits to observe customer installs and manufacturing procedures. In addition, each new employee is assigned a mentor to ensure continuing guidance and growth. Power also offers unique compensation models and incentives that reward employees beyond traditional salaries. From monthly company-wide sales competitions with opportunities to win highly coveted prizes, including trips to overseas destinations, iPads and theater tickets, to an annual holiday vacation paid for by Power, Power provides each employee with perks to keep them engaged and motivated throughout the year.

For more information on career opportunities at Power Home Remodeling Group, please visit the company's LinkedIn page or PowerHRG.com/Careers.

About Power Home Remodeling Group Family-owned since 1992, Power Home Remodeling Group is the nation'ssixth largesthome remodeling company with more than 1,200 employees and $135 million in sales. Headquartered in Chester, Pa., Power provides energy-saving and environmentally friendly exterior remodeling solutions to residents across the country, including Connecticut, Delaware, Georgia, Illinois, Maryland, Massachusetts, New Hampshire, New Jersey, New York, Pennsylvania, Virginia, and Washington, DC.AnInc. 5000company, Power has served more than 60,000 homeowners and is recognized as a home improvement leader by its two-time "Dealer of the Year" award fromWindow and Door Magazineand was named the top remodeler onProfessional Remodeler's 2012 Market Leaders Philadelphia list.For more information please visit PowerHRG.com, or follow the company on Twitter@PowerHRGand Facebook atfacebook.com/PowerHRG.

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Power Home Remodeling Group™ Named Top Workplace by Boston Globe

Home for sale in Gholson, TX | $270000
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Cleaning vinyl and concrete porches used as work areas.
Cleaning vinyl and concrete porches used as work areas needs to be done every few years using at least a water hose, making repairs and repainting.From:wmhoveysmithViews:18 3ratingsTime:11:06More inHowto Style

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Homes for Sale - 2601 SHAWNEE, Anderson, IN
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Homes for Sale - 2601 SHAWNEE, Anderson, IN - Video

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American Plumber WRC25HD20 Radial Flow Carbon Filter
http://www.waterfilters.net/American-Plumber-WRC25HD20-Carbon-Water-Filters.html The American Plumber WRC25HD20 water filter replacement cartridge is used to reduce chlorine taste and odor and sediment down to 25 microns. Radial Flow Carbon 25 Micron, 20" length. American Plumber WRC25HD20 water filters can be used in all big blue, big clear, big boy, and heavy-duty water filter housings that use 20" L x 4.5" W water filters. The American Plumber WRC25HD20 is made by Pentek (Pentair Inc.) and is the same filter as the Pentek RFC-20BB water filter.From:TheFilterDoctorViews:0 0ratingsTime:01:54More inHowto Style

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American Plumber WRC25HD Radial Flow Carbon Filter
http://www.waterfilters.net/American-Plumber-WRC25HD-Radial-Flow-Water-Filters.html The American Plumber WRC25HD water filter replacement cartridge is used to reduce chlorine taste and odor and sediment down to 25 microns. Radial Flow Carbon 25 Micron, 10" length. 9.75" L x 4.5" W; 25 Micron; Radial Flow Carbon; Chlorine Taste Odor Reduction. American Plumber WRC25HD water filters can be used in all big blue, big clear, big boy, and heavy-duty water filter housings that use 10" L x 4.5" W water filters. The American Plumber WRC25HD is made by Pentek (Pentair Inc.) and is the same filter as the Pentek RFC-BB water filter and Culligan RFC-BBS.From:TheFilterDoctorViews:0 0ratingsTime:01:51More inHowto Style

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American Plumber WRC25HD Radial Flow Carbon Filter - Video